BMG LabtechCorningDeerac logo Labcyte logoPromega logo

BMG Labtech, Corning, Promega, Labcyte and Deerac Fluidics are joint sponsors of a special workshop prior to the start of the SBS Annual Meeting. The workshop will be held on Sunday, April 6th at the America’s Center St. Louis from 9:30am -12:30pm in room 231. The Workshop title is:

Assay Optimization and Kinase Profiling in uHTS Format

Agenda

9:00AM-9:45AMContinental Breakfast and Informal Discussions

9:45AM-10:15AM - Advantages of Bioluminescence for uHTS Kinase Profiling
Sarah Shultz, MS, Applications Scientist, Promega

10:15AM-10:45AM - Moving to 1536-well plates with Echo®: Challenges and Solutions
Jean Shieh, Applications Specialist, Labcyte

10:45AM-11:00AM - Break

11:00AM-11:30AM - Low-Volume, High-Precision Dispensing for the HTS Environment
Aoife Gallagher, PhD, Head of Applications, Deerac Fluidics

11:30PM-12:00PMHigh Performance Luminescence Detection Using the PHERAstar HTS Multimode Microplate Reader
E.J. Dell, PhD, Application Scientist, BMG LABTECH, Inc.

12:00PM-12:30PM- Round Table Discussion

Successful kinase screening requires precise liquid handling, robust assays, reliable plates, and sensitive detection methods. With the increasing number of options available to the screener, it is a challenge to choose which are best suited for a particular application. This workshop demonstrates the complementarity of diverse technologies for a 1536-well kinase assay application.

Using a luminescent kinase technology platform from Promega, we demonstrate the optimization of kinase assays with the Deerac Fluidics Equator dispenser, using assay plates from Corning and with luminescent detection by BMG LABTECH's Pherastar. Following assay optimization, the Labcyte Echo® 555 liquid handler is used for ultra low-volume compound dispensing for screening and compound titration applications. Data show that miniaturization does not compromise quality when lower volume assays are required.

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